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Product Details
Application
Synonyms
Claudin-6, CLDN6
Source
Nanodisc Human Claudin-6 Protein, His,Twin-Strep Tag (CL6-H5286) is expressed from Baculovirus-Insect cells. It contains AA Ala 2 - Val 220 (Accession # P56747).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus and a twin strep tag at the C-terminus.
The protein has a calculated MW of 28.3 kDa. The protein migrates as 23-28 kDa under reducing (R) condition (SDS-PAGE).
*The isotype control of empty/mock nanodisc (Cat. No. APO-H51H3) is sold separately and not included in protein, you can follow the link for product information.
Structure
Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.
Synthesis Process
Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.
Purity
>90% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 12 months under sterile conditions.
ACRO Quality Management System
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Data Display
SDS-PAGE
Human Claudin-6 Protein, His,Twin-Strep Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Human Claudin-6 Protein, His,Twin-Strep Tag (Cat. No. CL6-H5286) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human Claudin-6 antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Anti-Claudin-6 Antibody, Human IgG1 captured on Protein A Chip can bind Human Claudin-6 Protein, His,Twin-Strep Tag (Cat. No. CL6-H5286) with an affinity constant of 1.08 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Evaluation of CAR expression
FACS Analysis of Anti-Claudin-6 CAR Expression
2e5 of anti-Claudin-6 (AE49-11) CAR-293 cells were stained with 100 μL of 10 μg/mL of Human Claudin-6 Protein, His,Twin-Strep Tag (Cat. No. CL6-H5286) and negative control protein respectively (Fig. A and B), FITC signal was used to evaluate the binding activity (QC tested).
ProtocolFACS Analysis of Non-specific binding to PBMCs
5e5 of PBMCs were stained with Human Claudin-6 Protein, His,Twin-Strep Tag (Cat. No. CL6-H5286) and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in PBMCs, and FITC signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Claudin 6
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
15 Details
- Highest R&D Stage：
Phase 2 Clinical
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