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Product Details
Application
Synonyms
Claudin-18.2, CLDN18, Claudin-18
Source
Nanodisc PE-Labeled Human / Cynomolgus Claudin18.2 Protein, His,Flag Tag (CL2-HP2D3) is produced via site-specific conjugation of PE to Human / Cynomolgus Claudin18.2 Protein, His,Flag Tag under optimal conditions with a proprietary technology. Human / Cynomolgus Claudin18.2 Protein, His,Flag Tag is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 200 (Accession # P56856-2). In the region Met 1 - Ala 200, the amino acid sequences of Human and Cynomolgus Claudin-18.2 are identical.
Predicted N-terminus: Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus and a Flag tag at the C-terminus. The protein has a calculated MW of 26.8 kDa.
*The isotype control of empty/mock nanodisc (Cat. No. APO-HP1E3) is sold separately and not included in protein, you can follow the link for product information.
Structure
Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.
Synthesis Process
Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Formulation
Supplied as 0.2 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Data Display
Bioactivity-ELISA
Immobilized PE-Labeled Human / Cynomolgus Claudin18.2 Protein, His,Flag Tag (Cat. No. CL2-HP2D3) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Chimeric Claudin-18.2 Antibody, Human IgG1 (Cat. No. CL2-M34) with a linear range of 0.0003-0.078 μg/mL (Routinely tested).
Protocol
Bioactivity-FACS
5e5 of Anti-Claudin18.2 CAR-293 cells were stained with 100 μL of 1:25 dilution (4 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human / Cynomolgus Claudin18.2 Protein, His,Flag Tag (Cat. No. CL2-HP2D3) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Claudin-18 splice variant 2
- Category：
- Listed Drugs Count：
1 Details
- Clinical Drugs Count：
82 Details
- Highest R&D Stage：
Approved
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