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Product Details
Synonyms
CD8A, CD8, Leu2, MAL, p32
Source
Human CD8 alpha, His Tag (CDA-H52H3) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 (Accession # P01732-1).
Predicted N-terminus: Ser 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 19.5 kDa. The protein migrates as 26-32 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Human CD8 alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CD8 alpha, His Tag (Cat. No. CDA-H52H3) is more than 95% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human CD8 alpha, His Tag (Cat. No. CDA-H52H3) at 5 μg/mL (100 μL/well) can bind Anti-CD8 alpha Antibody, Human IgG1 with a linear range of 1-2 ng/mL (QC tested).
Protocol
Immobilized Anti-CD8 alpha Antibody, Mouse IgG2a (clone: OKT8) at 1 μg/mL (100 μL/well) can bind Human CD8 alpha, His Tag (Cat. No. CDA-H52H3) with a linear range of 0.6-10 ng/mL (Routinely tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Cluster of differentiation 8
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
18 Details
- Highest R&D Stage：
Phase 3 Clinical
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