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Product Details
Synonyms
CD8 alpha & beta, CD8A & CD8B
Source
Biotinylated Cynomolgus CD8A&CD8B Heterodimer Protein, His,Avitag&Tag Free (CDA-C82W3) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Asp 182 & Leu 22 - Pro 170 (Accession # XP_065382910.1 & XP_005575417.1).
Predicted N-terminus: Ser 22 & Leu 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Biotinylated Cynomolgus CD8A&CD8B Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of CD8 alpha and CD8 beta, has a calculated MW of 25.4 kDa (CD8 alpha) and 21.1 kDa (CD8 beta). Subunit CD8 alpha is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit CD8 beta contains no tag at the C-terminus. The protein migrates as 30-32 kDa and 33-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Biotinylated Cynomolgus CD8A&CD8B Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Cynomolgus CD8A&CD8B Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDA-C82W3) is more than 95% and the molecular weight of this protein is around 50-75 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Anti-CD8 alpha Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD8A&CD8B Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDA-C82W3) with a linear range of 0.002-0.063 μg/mL (QC tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
T-cell surface glycoprotein CD8 beta chain
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
0 Details
- Highest R&D Stage：
Preclinical
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