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Product Details
Synonyms
CD3E & CD3D, CD3 delta & CD3 epsilon
Source
Human CD3E&CD3D Heterodimer Protein (CDD-H52Wa) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 | Phe 22 - Ala 105 (Accession # P07766-1 | P04234-1).
Predicted N-terminus: Asp 23 | Phe 22
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Human CD3E&CD3D Heterodimer Protein is produced by co-expression of CD3E and CD3D, has a calculated MW of 40.2 kDa (CD3E) and 37.3 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc tag and a polyhistidine tag at the C-terminus and subunit CD3D is fused with a human IgG1 Fc tag and a flag tag at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 45-60 kDa under reducing (R) condition, and 100-116 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE).
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
After reconstitution, this product is stable after storage at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human CD3E&CD3D Heterodimer Protein on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CD3E&CD3D Heterodimer Protein (Cat. No. CDD-H52Wa) is more than 85% and the molecular weight of this protein is around 80-90 kDa verified by SEC-MALS.
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Bioactivity-ELISA
Immobilized Human CD3E&CD3D Heterodimer Protein (Cat. No. CDD-H52Wa) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) with a linear range of 0.2-3 ng/mL (QC tested).
Protocol
Immobilized Human CD3E&CD3D Heterodimer Protein (Cat. No. CDD-H52Wa) at 1 μg/mL (100 μL/well) can bind UCHT1 with a linear range of 0.1-3 ng/mL (Routinely tested).
Protocol
Immobilized Human CD3E&CD3D Heterodimer Protein (Cat. No. CDD-H52Wa) at 1 μg/mL (100 μL/well) can bind SP34-2 with a linear range of 0.4-3 ng/mL (Routinely tested).
Protocol
Bioactivity-TR-FRET
In this TR-FRET assay, Streptavidin Protein Europium chelate (for TR-FRET) (Cat. No. FRT-TD09) is used as the Donor and Monoclonal Anti-Flag Tag Antibody FA-Labeled (for TR-FRET) (Cat. No. FRT-TA06) is used as the Acceptor. Human CD3E&CD3D Heterodimer Protein (Cat. No. CDD-H52Wa), at 10 ng/well and Biotinylated Human Kallikrein 2 Protein, His,Avitag (Cat. No. KA2-H82E3), at 5 ng/well, can bind to the Pasritamig with a linear detection range of 4-0.016 μg/mL (Routinely tested).
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