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Product Details
Synonyms
CD28, Tp44
Source
Human / Cynomolgus / Rhesus macaque CD28 Protein, His Tag (CD8-H52H3) is expressed from human 293 cells (HEK293). It contains AA Asn 19 - Pro 152 (Accession # P10747-1). In the region Asn 19 - Pro 152, the amino acid sequences of Human, Cynomolgus and Rhesus macaque CD28 are identical.
Predicted N-terminus: Asn 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 20.4 kDa. The protein migrates as 38-45 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human / Cynomolgus / Rhesus macaque CD28 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human / Cynomolgus / Rhesus macaque CD28 Protein, His Tag (Cat. No. CD8-H52H3) is more than 95% and the molecular weight of this protein is around 65-75 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human / Cynomolgus / Rhesus macaque CD28 Protein, His Tag (Cat. No. CD8-H52H3) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD28 Antibody, premium grade (Cat. No. CD8-M120b) with a linear range of 0.01-1 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Human B7-1, Fc Tag (Cat. No. B71-H5259) on Protein A Biosensor, can bind Human / Cynomolgus / Rhesus macaque CD28 Protein, His Tag (Cat. No. CD8-H52H3) with an affinity constant of 4.5 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
T-cell-specific surface glycoprotein CD28
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
31 Details
- Highest R&D Stage：
Phase 2 Clinical
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