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Product Details
Synonyms
CD70, CD27LG, TNFSF7, TNFSF7G, CD27L
Source
Mouse CD27 Ligand Protein, Mouse IgG2a Fc Tag (CDL-M5251) is expressed from human 293 cells (HEK293). It contains AA Gln 47 - Pro 195 (Accession # O55237-1).
Predicted N-terminus: Glu
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a mouse IgG2a Fc tag at the N-terminus.
The protein has a calculated MW of 43.5 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, 25 mM Arginine, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Mouse CD27 Ligand Protein, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human CD27, His Tag (Cat. No. CD7-H522b) at 1 μg/mL (100 μL/well) can bind Mouse CD27 Ligand Protein, Mouse IgG2a Fc Tag (Cat. No. CDL-M5251) with a linear range of 0.3-5 ng/mL (QC tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
CD70 antigen
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
37 Details
- Highest R&D Stage：
Phase 2 Clinical
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