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Product Details
Application
Synonyms
MS4A1, CD20, MS4A-1
Source
Nanodisc Biotinylated Cynomolgus CD20 Full Length Protein, His,Avitag (CD0-C82E4) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Pro 297 (Accession # M4ZHZ6-1 ).
Predicted N-terminus: Met 1
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 36.9 kDa. The protein migrates as 24-25 kDa and 40-41 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
*The isotype control of empty/mock nanodisc (Cat. No. APO-H81Q5) is sold separately and not included in protein, you can follow the link for product information.
Structure
Nanodisc membrane protein is wrapped by a synthetic phospholipid bilayer membrane structure, composed of membrane scaffold proteins (MSPs) and phospholipids.
Synthesis Process
Nanodisc reconstitution is a self-assembly process that starts from purified, detergent-solubilized membrane proteins and reconstitutes them into synthetic phospholipid bilayers stabilized by MSP1D1 membrane scaffold protein. This creates a stable, detergent-free environment that preserves native conformation and biological activity, with excellent homogeneity and full compatibility with cell-based assays such as FACS and binding assays in antibody discovery and screening (ELISA),etc.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>80% as determined by SDS-PAGE.
Formulation
Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Shipping and Storage
This product is supplied and shipped on dry ice.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Data Display
SDS-PAGE
Biotinylated Cynomolgus CD20 Full Length Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 80% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Biotinylated Cynomolgus CD20 Full Length Protein, His,Avitag (Cat. No. CD0-C82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Biosimilar Ofatumumab with a linear range of 0.039-0.625 μg/mL (QC tested).
Protocol
Immobilized Biotinylated Cynomolgus CD20 Full Length Protein, His,Avitag (Cat. No. CD0-C82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Biosimilar Obinutuzumab with a linear range of 0.04-1 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Rituximab biosimilar (Cat. No. CD0-M36)captured on Protein A Chip can bind Biotinylated Cynomolgus CD20 Full Length Protein, His,Avitag (Cat. No. CD0-C82E4) with an affinity constant of 22 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
B-lymphocyte antigen CD20
- Category：
- Listed Drugs Count：
35 Details
- Clinical Drugs Count：
132 Details
- Highest R&D Stage：
Approved
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Drug Candidate Licensing
