Collect Product
Product Details
Synonyms
CD19, B4, CVID3, MGC12802
Source
PE-Labeled Human CD19 (20-291), His Tag (CD9-HP2H3) is produced via site-specific conjugation of PE to Human CD19 (20-291), His Tag under optimal conditions with a proprietary technology. Human CD19 (20-291), His Tag is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291 (Accession # P15391-1).
Predicted N-terminus: Pro 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 33.7 kDa.
Conjugate
PE
Excitation Wavelength: 488 nm / 561 nm
Emission Wavelength: 575 nm
Application
Evaluation of anti-CD19 CAR expression by flow cytometry. Please note that this product is NOT compatible to streptavidin detection system.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Kunden haben auch angesehen
Data Display
FACS Analysis of Blood Samples
After Fc receptor blockade, whole blood only control (Figure A) and whole blood spiked with CAR-T cells (Figure B) were first stained with PE-Labeled Human CD19 (20-291) Protein, His Tag (Cat. CD9-HP2H3), following 7-AAD staining. The data were analyzed using FCS Express 7 software (Routinely tested). CAR-T cells were identified as PE-positive events within the live CD45+CD3+ (or CD45+) lymphocyte population. The proportion of CD45+CAR+ cells is around 20%, which is largely consistent with the theoretical spike-in value.
Protocol
Bioactivity-FACS
1e6 of the anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human CD19 (20-291), His Tag (Cat. No. CD9-HP2H3) (QC tested).
Protocol
User Reviews Publish Comment
P***y2023-06-20
LikesWe use this reagent to detect CD19 CAR on the cell surface by flow cytometry. We use it at the recommended dose and are very happy with the results.
- S*** 2023-02-01 LikesI run lentiviral titers at work and desperately needed a CD19 antibody due backorder. I stumbled upon Acro Bio's PE-CD19 abs and it worked wonders! When I first used it, there was higher CD19 expression than I typically see in my vectors. I performed a comparison study testing out various CD19 (some conjugated some not conjugated) antibodies. The Acro Bio antibody had the best performance! It showed greater binding than the usual CD19 antibody I use. After I conducted this study, my company set their heart's desire to Acro's CD19 antibody. We solely use this antibody for all our CD19 projects. AND its more affordable than many other CD19 antibodies on the market! This is such a phenomenal antibody! I highly recommend it!
- R***t2023-01-04 LikesThank you so much ACRO for your CD19 materials. I work in a CAR focused lab and the CD19 we get from you is instrumental to our operations. I work in immunology and this antibody is used in all my downstream flow assays to check the status of our novel CAR materials. I use it at least weekly, and the staining is easy and immaculate. My peaks are clear and I couldn't be happier.
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
B-lymphocyte antigen CD19
- Category：
- Listed Drugs Count：
14 Details
- Clinical Drugs Count：
435 Details
- Highest R&D Stage：
Approved
Contact Us
Drug Candidate Licensing
