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Product Details
Synonyms
CD19, B4, CVID3, MGC12802
Source
Human CD19 (20-291), Fc Tag, premium grade (CD9-H5251) is expressed from human 293 cells (HEK293). It contains AA Pro 20 - Lys 291 (Accession # P15391-1).
Predicted N-terminus: Pro 20
It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 56.6 kDa. The protein migrates as 70.0 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Sterility
Negative
Mycoplasma
Negative
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Human CD19 (20-291), Fc Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CD19 (20-291), Fc Tag, premium grade (Cat. No. CD9-H5251) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a at 1 μg/mL (100 μL/well) can bind Human CD19 (20-291), Fc Tag, premium grade (Cat. No. CD9-H5251) with a linear range of 0.1-8 ng/mL (QC tested).
Protocol
Immobilized Human CD19 (20-291), Fc Tag, premium grade (Cat. No. CD9-H5251) at 5 μg/mL (100 μL/well) can bind FMC63 (Mouse IgG2a) with a linear range of 0.6-5 ng/mL (Routinely tested).
Protocol
Immobilized Human CD19 (20-291), Fc Tag, premium grade (Cat. No. CD9-H5251) at 1 μg/mL, add increasing concentrations of Blinatumomab and then add HRP-Human CD3E & CD3D Heterodimer Protein, His Tag&Tag Free (Cat. No. CDD-HR2W3) at 1 μg/mL, with sensitivity of 34.21 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human CD19 (20-291), Fc Tag, premium grade (Cat. No. CD9-H5251) captured on CM5 chip via Anti-Human lgG Fc antibodies surface, can bind FMC63 MAb (Mouse lgG2a) with an affinity constant of 0.17 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bispecific T cell Engager (CD3 X CD19) captured on CM5 chip, can bind Human CD19 (20-291), Fc Tag, premium grade (Cat. No. CD9-H5251) with an affinity constant of 0.93 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human CD19 (20-291), Fc Tag, premium grade (Cat. No. CD9-H5251) on AHC Biosensor, can bind FMC63 MAb (Mouse lgG2a) with an affinity constant of 0.483 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Evaluation of CAR expression
FACS Analysis of anti-CD19 CAR Expression
293 cells were transfected with FMC63-scFv and RFP tag. 2e5 of the cells were first stained with B. Human CD19 (20-291), Fc Tag, premium grade (Cat. No. CD9-H5251, 3 µg/mL) and C. Human Fc Tag Protein Control, followed by FITC-conjugated Anti-human IgG Fc Antibody. A. Non-transfected 293 cells and C. Human Fc Tag Protein Control were used as negative control. RFP was used to evaluate CAR (anti-CD19-scFv) expression and FITC was used to evaluate the binding activity of Human CD19 (20-291), Fc Tag, premium grade (Cat. No. CD9-H5251).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
B-lymphocyte antigen CD19
- Category：
- Listed Drugs Count：
14 Details
- Clinical Drugs Count：
435 Details
- Highest R&D Stage：
Approved
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