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Product Details
Application
Synonyms
CCR5, CMKBR5, CD195, CC-CKR-5, CHEMR13
Source
Detergent Human CCR5, Flag,His Tag (CC5-H52D1) is expressed from human 293 cells (HEK293). It contains AA Asp 2 - Arg 223 & Glu 227 - Arg 319 (Accession # P51681-1 (C58Y, G163N, A233D, K303E)).
Predicted N-terminus: Asp
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a flag tag at the N-terminus and a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 46.0 kDa. The protein migrates as 40-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
*The DDM/CHS buffer (Cat. No. DC-11) is sold separately and not included in protein, you can follow the link for product information.
Structure
Each full-length transmembrane protein is encapsulated by detergent micelle.
Synthesis Process
Through mild detergent micelle extraction, dedicated solubilization, and stringent affinity purification, we maximally retain the native transmembrane conformation of full-length transmembrane proteins throughout downstream purification. This workflow yields purified, detergent-solubilized proteins free of unrelated membrane impurities and exogenous scaffold proteins, with exceptional purity, accurate quantitation, and well-preserved biological activity—ideal for immunization, binding assays in antibody discovery and screening (ELISA) and affinity testing (SPR and BLI), etc.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>85% as determined by SDS-PAGE.
Formulation
This product is not suitable for cell based experiments due to cytotoxicity of DDM.
DDM and CHS are INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove DDM and CHS.
DDM/CHS buffer (DC-11) is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, DDM, CHS, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- The product MUST be stored at -70°C or lower upon receipt;
- -70°C for 3 months under sterile conditions.
ACRO Quality Management System
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Data Display
SDS-PAGE
Human CCR5, Flag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human CCR5, Flag,His Tag (Cat. No. CC5-H52D1) at 10 μg/mL (100 μL/well) can bind Anti-Human CCR5 Antibody with a linear range of 0.02-1.25 μg/mL (QC tested).
Protocol
Bioactivity-SPR
Human CCR5, Flag,His Tag (Cat. No. CC5-H52D1) captured on CM5 chip via anti-His antibody can bind Anti-CCR5 antibody (Human IgG1) with an affinity constant of 0.0737 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore T200) (Routinely tested).
Protocol
Purified NA/LE Mouse Anti-Human CD195 (Clone: 2D7) (Mouse IgG2a) captured on CM5 chip via anti-mouse antibodies surface can bind Human CCR5, Flag,His Tag (Cat. No. CC5-H52D1) with an affinity constant of 7.51 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore T200) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
C-C chemokine receptor type 5
- Category：
- Listed Drugs Count：
1 Details
- Clinical Drugs Count：
13 Details
- Highest R&D Stage：
Approved
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