Human CCL1 / I-309 Protein, Fc Tag (MALS verified)

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Cat. No. / Size
Price
Qty
CC1-H5254-20ug
$185.00
CC1-H5254-50ug
$370.00
CC1-H5254-1mg (500ug X 2)
$3400.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    CCL1, I-309, C-C motif chemokine 1

  • Source

    Human CCL1, Fc Tag (CC1-H5254) is expressed from human 293 cells (HEK293). It contains AA Lys 24 - Lys 96 (Accession # P22362-1).

    Predicted N-terminus: Lys 24

    Request for sequence
  • Molecular Characterization

    CCL1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 34.9 kDa. The protein migrates as 40-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CCL1 SDS-PAGE

    Human CCL1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    CCL1 SEC-MALS

    The purity of Human CCL1, Fc Tag (Cat. No. CC1-H5254) is more than 95% and the molecular weight of this protein is around 68-88 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     CCL1 ELISA

    Immobilized Human CCR8 Protein, Flag,His Tag&Twin-Strep Tag (Cat. No. CC8-H52D6) at 5 μg/mL (100 μL/well) can bind Human CCL1, Fc Tag (Cat. No. CC1-H5254) with a linear range of 0.01-0.625 μg/mL (Routinely tested).

    Protocol

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Background

Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL1, a member of the CC subfamily, is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine (C-C motif) receptor 8.

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