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Product Details
Synonyms
CADM1, BL2, IGSF4, IGSF-4, IGSF4A, NECL2, Necl-2, RA175, ST17, SYNCAM, TSLC1, sTSLC-1, sgIGSF, synCAM1, DKFZp686F1789, MGC149785, MGC51880
Source
Human CADM1, His Tag (CA1-H5225) is expressed from human 293 cells (HEK293). It contains AA Gln 45 - His 374 (Accession # Q9BY67).
Predicted N-terminus: Gln 45
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 37.86 kDa. The protein migrates as 50 kDa and 55-85 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to different glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human CADM1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Cynomolgus CRTAM, Fc Tag (Cat. No. CRM-C5255) at 5 μg/mL (100 μL/well) can bind Human CADM1, His Tag (Cat. No. CA1-H5225) with a linear range of 0.6-5 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Human CRTAM, His Tag (Cat. No. CRM-H52H3) on AR2G Biosensor, can bind Human CADM1, His Tag (Cat. No. CA1-H5225) with an affinity constant of 51.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Cynomolgus CRTAM, Fc Tag (Cat. No. CRM-C5255) on Protein A Biosensor, can bind Human CADM1, His Tag (Cat. No. CA1-H5225) with an affinity constant of 37.7 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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