FITC-Labeled Human Cadherin-17 / CDH17 (23-787) Protein, His Tag

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Cat. No. / Size
Price
Qty
CA7-HF2H3-25ug
$330.00
CA7-HF2H3-200ug
$2245.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Cadherin-17, CDH17, HPT-1, LI-cadherin

  • Source

    FITC-Labeled Human Cadherin-17 (23-787) Protein, His Tag (CA7-HF2H3) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Met 787 (Accession # Q12864-1). It is the FITC labeled form of Human Cadherin-17 Protein, His Tag (Active) (Cat. No. CA7-H52H4).

    Predicted N-terminus: Gln 23

    Request for sequence
  • Molecular Characterization

    Cadherin-17 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 86.9 kDa. The protein migrates as 95-110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling

    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular sieve treatment during purification process.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Cadherin-17 SDS-PAGE

    FITC-Labeled Human Cadherin-17 (23-787) Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     Cadherin-17 ELISA

    Immobilized FITC-Labeled Human Cadherin-17 (23-787) Protein, His Tag (Cat. No. CA7-HF2H3) at 1 μg/mL (100 μL/well) can bind various dilution ratio of Anti-Cadherin 17 Antibody (QC tested).

    Protocol
  • Bioactivity-SPR

     Cadherin-17 SPR

    Anti-Cadherin 17 Antibody captured on CM5 chip via anti-mouse antibodies surface can bind FITC-Labeled Human Cadherin-17 (23-787) Protein, His Tag (Cat. No. CA7-HF2H3) with an affinity constant of 3.11 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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Background

Cadherin-17, also known as liver-intestine (LI) Cadherin, belongs to the cadherin family of calcium-dependent cell adhesion molecules. In vivo studies showed CDH17 knockout resulted in apoptotic PC tumor death through activating caspase-3 activity. Taken together, CDH17 functions as an oncogenic molecule critical to PC growth by regulating tumor apoptosis signaling pathways and CDH17 could be targeted to develop an anti-PC therapeutic approach.

Recent Advances

 
Drug Development Progress
  • English Name:

    Cadherin-17

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    17 Details

  • Highest Development Stage:

    Phase 2 Clinical

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  • Background