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Product Details
Synonyms
B2M
Source
Human B2M, His Tag (B2M-H5225) is expressed from human 293 cells (HEK293). It contains AA Ile 21 - Met 119 (Accession # P61769-1).
Predicted N-terminus: Ile 21
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 12.6 kDa. The protein migrates as 14 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Human B2M, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
User Reviews Publish Comment
K***y2024-10-09
LikesThe product was used to develop and benchmark in-solution binding kinetics of B-2-microglobulin with anti-B-2-microglobulin mAb. We received the product in lyophilized form with clear reconstitution instructions. It is appreciated that the product comes with detailed CoA listing all specific details needed like molecular weight, SDSPAGE data and buffer composition with clear information on additive components. This helped us properly label the protein and perform subsequent binding analysis using Flow Induced Dispersion Analysis (FIDA) for determination of in-solution binding kinetics (kon & koff) parameters.
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Beta-2-microglobulin
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
0 Details
- Highest R&D Stage：
Preclinical
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