Save Product
Product Details
Synonyms
B7-H7, HHLA2, B7 Homolog 7
Source
Biotinylated Human B7-H7, His,Avitag (B77-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Asn 344 (Accession # Q9UM44-1).
Predicted N-terminus: Ile 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 40.7 kDa. The protein migrates as 50-66 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Biotinylated Human B7-H7, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human B7-H7, His,Avitag (Cat. No. B77-H82E3) is more than 95% and the molecular weight of this protein is around 48-73 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human B7-H7, His,Avitag (Cat. No. B77-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human CD28H, Fc Tag (Cat. No. CDH-H5251) with a linear range of 0.04-3 ng/mL (QC tested).
Protocol
Immobilized Human CD28H, Fc Tag (Cat. No. CDH-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Human B7-H7, His,Avitag (Cat. No. B77-H82E3) with a linear range of 0.039-1.25 μg/mL (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Drug Development Progress- English Name：
HERV-H LTR-associating protein 2
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 1 Clinical
Contact Us
Drug Candidate Licensing
