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Product Details
Synonyms
B7-H4, VTCN1, B7S1, B7h.5
Source
Cynomolgus / Rhesus macaque B7-H4 Protein, His Tag (B74-C52H9) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Ala 258 (Accession # F7B770-1). In the region Phe 29 - Ala 258, the amino acid sequences of Cynomolgus and Rhesus macaque B7-H4 are identical.
Predicted N-terminus: Phe 29
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 27.2 kDa. The protein migrates as 45-60 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Cynomolgus / Rhesus macaque B7-H4 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Cynomolgus / Rhesus macaque B7-H4 Protein, His Tag (Cat. No. B74-C52H9) is more than 95% and the molecular weight of this protein is around 42-57 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Cynomolgus / Rhesus B7-H4, His Tag (Cat. No. B74-C52H9) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human B7-H4 Antibody, Human IgG1 with a linear range of 0.1-8 ng/mL (QC tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
V-set domain-containing T-cell activation inhibitor 1
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
11 Details
- Highest R&D Stage：
Phase 3 Clinical
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