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Product Details
Synonyms
ICOSLG, B7-H2, B7H2, B7RP-1, B7RP1, CD275, GL50, ICOS-L, ICOSL, LICOS, ICOS ligand
Source
Biotinylated Cynomolgus B7-H2 Protein, His,Avitag (B72-C82E3) is expressed from human 293 cells (HEK293). It contains AA Asp 19 - Thr 256 (Accession # XP_005548617.1).
Predicted N-terminus: Asp 19
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 30.3 kDa. The protein migrates as 43-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Cynomolgus B7-H2 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Cynomolgus B7-H2 Protein, His,Avitag (Cat. No. B72-C82E3) is more than 90% and the molecular weight of this protein is around 40-60 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human ICOS, Fc Tag (Cat. No. ICS-H5258) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus B7-H2 Protein, His,Avitag (Cat. No. B72-C82E3) with a linear range of 0.05-1 ng/mL (QC tested).
Protocol
Immobilized Prezalumab at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus B7-H2 Protein, His,Avitag (Cat. No. B72-C82E3) with a linear range of 0.06-2 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
ICOS ligand
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
1 Details
- Highest Development Stage：
Phase 2 Clinical
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