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Product Details
Synonyms
ASGP-R 1, ASGPR 1, C-type lectin domain family 4 member H1, Hepatic lectin H1, HL-1
Source
Human ASGR1, Fc Tag (AS1-H5253) is expressed from human 293 cells (HEK293). It contains AA Gln 62 - Leu 291 (Accession # P07306-1).
Predicted N-terminus: Pro
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 52.8 kDa. The protein migrates as 65-75 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Human ASGR1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human ASGR1, Fc Tag (Cat. No. AS1-H5253) is more than 85% and the molecular weight of this protein is around 105-125 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human ASGR1, Fc Tag (Cat. No. AS1-H5253) at 1 μg/mL (100 μL/well) can bind Mouse Anti-Human ASGR1 antibody-4G5, Mouse IgG2b|Mouse Kappa with a linear range of 0.06-1 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Human ASGR1, Fc Tag (Cat. No. AS1-H5253) on Protein A Biosensor, can bind SARS-CoV-2 S1 protein NTD, His Tag (Cat. No. S1D-C52H6) with an affinity constant of 401 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human ASGR1, Fc Tag (Cat. No. AS1-H5253) on Protein A Biosensor, can bind SARS-CoV-2 S1 protein with an affinity constant of 4.09 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human ASGR1, Fc Tag (Cat. No. AS1-H5253) on Protein A Biosensor, can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 686 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Asialoglycoprotein receptor 1
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
1 Details
- Highest R&D Stage：
Phase 2 Clinical
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