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Product Details
Synonyms
Activin receptor type IIA, ACTRIIA
Source
Mouse Activin RIIA Protein, His Tag (ACA-M52H3) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Pro 135 (Accession # P27038-1).
Predicted N-terminus: Ala 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 15.3 kDa. The protein migrates as 25-35 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Mouse Activin RIIA Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Mouse Activin RIIA Protein, His Tag (Cat. No. ACA-M52H3) was greater than 95% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Mouse Activin RIIA Protein, His Tag (Cat. No. ACA-M52H3) at 1 μg/mL (100 μL/well) can bind Bimagrumab with a linear range of 0.05-1 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Mouse Activin RIIA Protein, His Tag (Cat. No. ACA-M52H3) capture on NTA-Series S sensor chip can bind Human Activin A Protein, Tag Free (Cat. No. ACA-H421b) with an affinity constant of 83.8 pM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Activin receptor type-2A
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
4 Details
- Highest R&D Stage：
Phase 2 Clinical
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