Product Details
Product Overview
Lp(a) (Lipoprotein(a)) is a unique and clinically important lipoprotein closely linked to cardiovascular risk. A lipoprotein particle made of an LDL-like core (contains ApoB-100) plus an additional protein called Apolipoprotein(a) (Apo(a)). Human Lipoprotein (a) ELISA Kitis a ready-to-use immunoassay kit, specifically designed to quantitate natural human Lipoprotein (a) that is present in biological samples, such as serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteLipoprotein (a)Format96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity150 pg/mLRange218.75 pg/mL-14000 pg/mLAssay Time2.75 hrSample volume50 μLSample TypeFor the quantitative determination of human Lipoprotein (a) in serum, plasma and cell supernatant.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
This kit is specifically designed for the accurate quantitation of native human Lp(a) from cell culture supernates, serum and plasma. The standard is a recombinant human Apo(a) protein, and the antibodies specifically recognize the Apo(a).
It is for research use only.
Workflow

Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA264-C01Pre-coated Anti-Lipoprotein (a) Antibody Microplate1 plateCEA264-C02Human Lipoprotein (a) Standard380 ng×2CEA264-C03Biotin-Anti-Lipoprotein (a) Antibody Con. Solution100 μLCEA264-C04Biotin-Antibody Dilution Buffer8 mLCEA264-C05Streptavidin-HRP Con. Solution500 μLCEA264-C06Streptavidin-HRP Dilution Buffer15 mLCEA264-C0720× Washing Buffer50 mLCEA264-C08Sample Dilution Buffer15 mL×2CEA264-C09Substrate Solution12 mLCEA264-C10Stop Solution6 mLACRO Quality Management System
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Data Display
Typical Data
Please refer to DS document for the assay protocol.

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of Lipoprotein (a) is less than 150 pg/mL.
Sample Values
43 healthy serum samples were evaluated for the concentrations of human Lipoprotein (a) in assay. The sample detection rate is 100%. The mean value is 5.206 μg/mL, and the range is from 0.495 to 17.815 μg/mL.

Intra-Assay Statistics
Eight replicates of each of three samples containing different Lipoprotein (a) concentrations were tested in one assay. Acceptable criteria: CV<10%.

Inter-Assay Statistics
Three samples containing different concentrations of Lipoprotein (a) were tested in independent assays. Acceptable criteria: CV<15%.

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