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Product Details
Product Overview
Human IL-23 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human IL-23 that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteIL-23Format96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity6 pg/mLRange7.5 pg/mL-480 pg/mLAssay Time3.75 hrSample volume100 μL (diluted)Sample TypeFor the quantitative determination of human IL-23, plasma and cell supernatant.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human IL-23 in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA093-C01Pre-coated Anti-IL-23 Antibody Microplate1 plateCEA093-C02Human IL-23 Standard500 ng/mL×2CEA093-C03Biotin-Anti- IL-23 Antibody Con. Solution100 μLCEA093-C04Biotin-Antibody Dilution Buffer15 mLCEA093-C05Streptavidin-HRP Con. Solution500 μLCEA093-C06Streptavidin-HRP Dilution Buffer15 mLCEA093-C0720× Washing Buffer50 mLCEA093-C08Sample Dilution Buffer15 mL×2CEA093-C09Substrate Solution12 mLCEA093-C10Stop Solution6 mLACRO Quality Management System
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Data Display
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of IL-23 is less than 6 pg/mL.
Sample Values
Human monocyte-derived macrophages (MDM) were obtained from PBMCs and attached monocytes were cultured in RPMI supplemented with 10% fetal bovine serum, 100 U/mL penicillin, 100 μg/mL streptomycin sulfate, and 100 ng/mL of recombinant human M-CSF for 5 days. Cells were treated with 1 μg/mL LPS and 40 ng/mL of recombinant human IFN-γ overnight.
Intra-Assay Statistics
Eight replicates of each of 3 samples containing different IL-23 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of IL-23 were tested in independent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant IL-23 was spiked into 3 human serum samples, and then analyzed. The average recovery of IL-23 for serum samples is 94.68%.
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