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Product Details
Product Overview
Mouse IL-1β ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant mouse IL-1β that is present in biological samples, such as serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteMouse IL-1βFormat96-wells plate breakable into 12 x 8 wells stripsReactivityMouseSensitivity3 pg/mLAssay Time2.75 hrSample volume50 μLRange9.4 pg/mL-600 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.NIBSC Code93/668Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant mouse IL-1β in serum, plasma and cell culture supernatants.
This Assay has been anchored to NlBSC/WHO standards (Code: 93/668), ELISA Kit: NIBSC/WHO Mass Conversion Factor is 1.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA250-C01Pre-coated Anti-mouse IL-1β Antibody Microplate1 plate (8×12 strips)CEA250-C02Mouse IL-1β Standard6 ng×2CEA250-C03Biotin-Anti-mouse IL-1β Antibody Con. Solution100μLCEA250-C04Biotin-Antibody Dilution Buffer8 mLCEA250-C05Streptavidin-HRP Con. Solution500 μLCEA250-C06Streptavidin-HRP Dilution Buffer15 mLCEA250-C0720×Washing Buffer50 mLCEA250-C08Sample Dilution Buffer15 mL×2CEA250-C09Substrate Solution12 mLCEA250-C10Stop Solution6 mLACRO Quality Management System
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Data Display
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of mouse IL-1β is less than 3 pg/mL.
Intra-Assay Statistics
Ten replicates of each of three samples containing different mouse IL-1β concentra-tions were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of mouse IL-1β were tested in in-dependent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant mouse IL-1β was spiked into three mouse serum samples, and then analyzed. The average recovery of IL-1β for serum samples is 98.7%.
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