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Product Details
FAB
- Comprehensive validation - Rigorously validated through comprehensive methodology to ensure reliable and accurate results.
- Simple and fast operation - No complicated washing steps, significantly reducing time.
- High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.
- Accurate and reliable results - High sensitivity with minimal matrix effects.
- High throughput capability - Support multiple product specifications, ideal for high-throughput screening.
- Fast completion - Results in just 2 hours.
Product Overview
Human IFN-gamma Detection kit (TR-FRET) can detect the human IFN-gamma in homogeneous system within 2 hours, it is highly sensitive, short detection time, easy to use and possesses high-throughput screening capability. Compatible with microplate formats, this kit supports high-throughput screening workflows, enabling efficient large-scale sample analysis. This kit is specifically designed for the accurate quantitation of human IFN-gamma from cell culture supernatant, serum and plasma.
Product Specifications
Assay TypeSandwich-TR-FRETAnalyteHuman IFN-gammaReactivityHumanRegulatory StatusRUOSensitivityLOQ=46.64 pg/mLStandard Curve Range46.64 pg/mL-4000 pg/mLAssay Time2 hrSuitable Sample TypeFor human Human IFN-gamma detection in human serum, human plasma,cell culture supernatantsSample volume16 μLElevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Workflow
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeFRTC01-C01Anti-Human IFN-γ Antibody Europium-chelate100 tests/500 testsFRTC01-C02FA Labeled Anti-Human IFN-γ Antibody100 tests/500 testsFRTC01-C03Human IFN-γ Standard20 μg/100 tests & 500 testsDB-04TR-FRET Sample Dilution Buffer, pH7.450 mL/100 tests & 500 testsDB-05TR-FRET Detection Buffer, pH7.450 mL/100 tests & 500 testsACRO Quality Management System
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Data Display
Typical Data
Please refer to DS document for the assay protocol.
Standard curve generated in TR-FRET Sample Dilution Buffer, pH7.4 (DB-04).
Dilution Linearity
To evaluate the linearity of the assay, samples spiked with high-concentration human IFN-gamma in various matrices were prepared. With DB-04 (TR-FRET Sample Dilution Buffer, pH 7.4) as the surrogate matrix, standard curves were generated and the spiked samples were subjected to serial gradient dilution, followed by the quantification of the spiked samples.
Intra-Assay Statistics
Five samples with known concentrations were tested repeatedly 16 times within a single analytical batch to assess within-batch precision and accuracy.
Inter-Assay Statistics
Five samples with known concentrations were tested repeatedly across three independent analytical batches to evaluate between-batch precision and accuracy.
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