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Product Overview
Human EGF ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human EGF that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteEGFFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity<35 pg/mLAssay Time2 hr 45 minSample volume50 μLRange62.5 pg/mL-8000 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.NIBSC Code91/530Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human EGF in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA080-C01Pre-coated Anti-EGF Antibody Microplate1 plateCEA080-C02Human EGF Standard7.5 μg×2CEA080-C03Biotin-Anti-EGF Antibody Con. Solution100 μLCEA080-C04Biotin-Antibody Dilution Buffer8 mLCEA080-C05Streptavidin-HRP Con. Solution500 μLCEA080-C06Streptavidin-HRP Dilution Buffer15 mLCEA080-C0720×Washing Buffer50 mLCEA080-C08Sample Dilution Buffer15 mL×2CEA080-C09Substrate Solution12 mLCEA080-C10Stop Solution6 mLACRO Quality Management System
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Data Display
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of EGF is less than 35 pg/mL.
Intra-Assay Statistics
Ten replicates of each of three samples containing different EGF concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of EGF were tested in independent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant EGF was spiked into three human serum samples, and then analyzed. The average recovery of EGF for serum samples is 105%.
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Drug Development Progress- English Name：
Epidermal growth factor
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- Highest R&D Stage：
Phase 2 Clinical
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