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Product Details
Product Overview
Human CCL5/RANTES ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human CCL5/RANTES that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteCCL5/RANTESFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity15.63 pg/mLAssay Time1.75 hrSample volume50 μLRange15.63 pg/mL-1000 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.NIBSC Code92/520Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human CCL5/RANTES in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA088-C01Pre-coated Anti-CCL5 Antibody Microplate1 plateCEA088-C02Human CCL5 Standard500 μLCEA088-C03Biotin-Anti-CCL5 Antibody Con. Solution100 μLCEA088-C04Biotin-Antibody Dilution Buffer8 mLCEA088-C05Streptavidin-HRP Con. Solution500 μLCEA088-C06Streptavidin-HRP Dilution Buffer15 mLCEA088-C0720×Washing Buffer50 mLCEA088-C082×Sample Dilution Buffer15 mL×2CEA088-C09Substrate Solution12 mLCEA088-C10Stop Solution6 mLACRO Quality Management System
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Data Display
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of CCL5/RANTES is less than 15.63 pg/mL.
Sample Values
Serum Sample: Ten healthy serum samples were evaluated for the concentrations of human CCL5/RANTES in assay.
Intra-Assay Statistics
Ten replicates of each of four samples containing different CCL5/RANTES concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Five samples containing different concentrations of CCL5/RANTES were tested in independent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant CCL5/RANTES was spiked into three human serum samples, and then analyzed. The average recovery of CCL5/RANTES for serum samples is 92.5%.
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Frontier Progress
Drug Development Progress- English Name：
C-C motif chemokine 5
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
2 Details
- Highest R&D Stage：
Phase 2 Clinical
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