Product Details
Source
Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) is a recombinant mouse monoclonal antibody expressed in HEK293 cells.
Antibody Type
Recombinant Monoclonal
Clone
6F2F7-2
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Immunogen
Trastuzumab
Specificity
Recognizes Trastuzumab specifically.
Application
ApplicationRecommended UsageELISA1:200-1:2000Purification
Protein A purified / Protein G purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in Tris (pH 7.5) containing 100 mM Glycine, 25 mM Arginine and 150 mM NaCl, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 12 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Data Display
Bioactivity-ELISA

Immobilized Trastuzumab at 1 μg/mL (100 μL/well) can bind Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) (Cat. No. TRB-Y5b) with a linear range of 0.19-6.25 ng/mL (QC tested).
Protocol
Serial dilutions of Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) ( Cat. No. TRB-Y5b) was detected by Biotinylated Human Her2, His,Avitag (Cat. No. HE2-H82E2) with a half maximal inhibitory concentration (IC50) of 7.02 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR

Trastuzumab captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Anti-Trastuzumab Antibody, Mouse IgG1 (6F2F7-2) (Cat. No. TRB-Y5b) with an affinity constant of 0.915 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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