Monoclonal Anti-HRSV F Antibody, Human IgG1 (AM14) (MALS verified)

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Cat. No. / Size
Price
Qty
HRF-S367-100ug
$415.00
HRF-S367-1mg (500ug X 2)
$2735.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Monoclonal Anti-HRSV F Antibody, Human IgG1 (AM14) is a recombinant chimeric monoclonal antibody expressed in CHO, which combines the variable region of a mouse monoclonal antibody with human constant domain.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    AM14

  • Isotype

    Human IgG1, Kappa

  • Host Species

    Mouse

  • Reactivity

    Virus

  • Specificity

    This product is a specific antibody specifically reacts with HRSV F.

  • Application

    Application
    Recommended Usage
    ELISA
    1:600-1:6000
  • Cross Verification

    This product can cross in Elisa with
    HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7).
    This product No cross-reactivity in ELISA with
    HRSV (B) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H8).
    HRSV (B) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H9).
    HRSV (A) Post-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H6).

  • Purification

    Protein A purified / Protein G purified.

  • Aggregation

    Less than 5%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     Prefusion glycoprotein F0/pre-F protein (RSV) ELISA

    Immobilized Monoclonal Anti-HRSV F Antibody, Human IgG1 (AM14) (Cat. No. HRF-S367) at 1 μg/mL (100 μL/well) can bind HRSV (A) Pre-fusion glycoprotein F0, His Tag (Cat. No. RSF-V52H7) with a linear range of 0.2-16 ng/mL (QC tested).

    Protocol

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Background

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. The RSV fusion glycoprotein (RSV F) is the principal target of RSV neutralizing antibodies in human sera. The RSV F is a type I viral fusion protein synthesized as inactive, single-chain polypeptides that assemble into trimers. RSV F fuses the viral and host cell membranes by irreversible protein refolding from the labile prefusion conformation to the stable post-fusion conformation. Antibody AM14 has a uniquely trimer-specific and and neutralizing activity. It recognizes a quaternary epitope that spans two protomers and includes a region that undergoes extensive conformational changes in the pre- to postfusion F transition. The specificity of the potent RSV neutralizing antibody AM14 makes it a useful reagent for probing or isolating the cleaved trimeric state of prefusion F.

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