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Alexa Fluor™ 647-Labeled Human IgG Antibody Isotype Control (mAb, carrier free, MALS verified)

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Product Number/Spec
Price
Menge
DNP-A904P1-25ug
$150.00
DNP-A904P1-200ug
$665.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Source

    Alexa Fluor 647-Labeled Human IgG Antibody Isotype Control (mAb) is a recombinant chimeric monoclonal antibody expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.

  • Antibody Type

    Recombinant Monoclonal

  • Isotype

    Human IgG1, Kappa

  • Host Species

    Human

  • Conjugate

    AF647

    Excitation Wavelength: 640 nm

    Emission Wavelength: 672 nm

  • Specificity

    Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins, which is normally not present in Humans or animals. Therefore, DNP-A904P1 does not react with any antigen of Human or animal cells.

  • Application

    Application
    Recommended Usage
    ELISA
    1:2000-1:20000
  • Purification

    Protein A purified / Protein G purified.

  • Aggregation

    Less than 10%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • Bioactivity-ELISA

     IgG Control ELISA

    Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Alexa Fluor 647-Labeled Human IgG Antibody Isotype Control (mAb) (Cat. No. DNP-A904P1) with a linear range of 0.08-1 ng/mL (QC tested).

    Protocol

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Publish Comment

Background Introduction

Fluorescently or biotin labeled isotype controls are used to assess non specific staining and autofluorescence in flow cytometry, ELISA, and imaging assays. By recognizing DNP, a hapten absent in biological samples, these controls provide a reliable baseline to distinguish background fluorescence or signal from true antigen specific binding.

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