Monoclonal Anti-Mumps virus HN Antibody, Human IgG1 (7D12) (MALS verified)

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Cat. No. / Size
Price
Qty
HNN-M701-100ug
$415.00
HNN-M701-1mg (500ug X 2)
$2735.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Monoclonal Anti-Mumps virus HN Antibody, Human IgG1 (7D12) is a recombinant chimeric monoclonal antibody expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    7D12

  • Isotype

    Human IgG1, Kappa

  • Host Species

    Mouse

  • Reactivity

    Virus

  • Immunogen

    Recombinant Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (HNN-M52H3) is expressed from human 293 cells

  • Specificity

    Specifically recognizes Mumps virus (strain Miyahara vaccine) (MuV) HN.

  • Application

    Application
    Recommended Usage
    ELISA
    1:8000-1:80000
  • Purification

    Protein A purified / Protein G purified.

  • Aggregation

    Less than 5%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     Hemagglutinin-neuraminidase/HN (MuV) ELISA

    Immobilized Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (Cat. No. HNN-M52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Mumps virus HN Antibody, Human IgG1 (7D12) (Cat. No. HNN-M701) with a linear range of 0.1-1 ng/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     Hemagglutinin-neuraminidase/HN (MuV) SPR

    Monoclonal Anti-Mumps virus HN Antibody, Human IgG1 (7D12) (Cat. No. HNN-M701) captured on Protein A Chip can bind Mumps virus (strain Miyahara vaccine) (MuV) HN Protein, His Tag (Cat. No. HNN-M52H3) with an affinity constant of 0.474 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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Background

The two surface glycoproteins of the mumps virus are the hemagglutinin-neuraminidase (HN) and Fusion proteins. These glycoproteins are essential for viral entry to host cells, and the spread of newly formed virions. The HN protein is a 582-amino acid structural glycoprotein of the mumps virus. HN is a type II membrane protein in which the N terminus is oriented towards the cytoplasm and the C terminus is extracellular.The HN protein exhibits both hemagglutinin and neuraminidase properties and is critical for membrane fusion and viral entry into host cells.

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