Product Details
Source
Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells, which provides higher batch consistency and long term security of supply.
Antibody Type
Recombinant Monoclonal
Clone
5B5
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Reactivity
Human
Immunogen
Purified Human CD71 Protein
Conjugate
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
Specificity
This product is a specific antibody specifically reacts with CD71 protein.
Application
Flow Cytometry (Evaluation of the expression of CD71 on Human cells).
Recommended Dilution
1:50
Isotype Control
The isotype control is sold separately; please refer to Cat. No. DNP-FM1A1 for product information.
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution.
- 2-8 °C for 12 months after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Performance Data
Bioactivity-FACS

Flow cytometric analysis of HEK293/Human Transferrin R Stable Cell Line (ACROBiosystems, Cat. No. CHEK-ATP089) staining with FITC-Labeled Monoclonal Anti-CD71 Antibody, Mouse IgG1 (5B5) (Cat. No. CD1-FMY21) at 1:50 dilution (2 μL of the stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. FITC signal was used to evaluate the binding activity (QC tested).
Protocol
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