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PE-Labeled Monoclonal Anti-Human CD45RO Antibody, Mouse IgG2a (UCHL1) (0.03% Proclin)

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Product Number/Spec
Price
Menge
FABm021-01-200tests (50tests X 4)
$200.00
ETA of in-stock products:2 business days
Gesamtanzahl Product Amount$ 0

Product Details

  • Clone

    UCHL1

  • Isotype

    Mouse IgG2a, Kappa

  • Host Species

    Mouse

  • Reactivity

    Human

  • Immunogen

    Purified Human CD45RO Protein

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Specificity

    This product is a specific antibody specifically reacts with CD45RO protein.

  • Application

    Flow Cytometry (Evaluation of the expression of CD45RO on Human cells).

  • Recommended Dilution

    1:20

  • Isotype Control

    The isotype control is sold separately; please refer to Cat. No. DNP-PM487 for product information.

  • Form

    Liquid

  • Formulation

    Supplied as 0.2 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Shipping

    Liquid product is shipped at blue ice.

  • Storage

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. Store at 2-8 °C for 12 months.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Data Display

  • Bioactivity-FACS

     CD45RO FACS

    Flow cytometric analysis of Jurkat staining with PE-Labeled Monoclonal Anti-Human CD45RO Antibody, Mouse IgG2a (UCHL1) (Cat. No. FABm021-01) at 1:20 dilution (5 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. PE signal was used to evaluate the binding activity (QC tested).

    Protocol
  • Compared Data

     CD45RO COMPARED_DATA

    Flow cytometric analysis of Jurkat staining with PE-Labeled Monoclonal Anti-Human CD45RO Antibody. PE signal was used to evaluate the binding activity of Anti-Human CD45RO Antibody. The biological activity level of ACRO is superior to competitor BL (Routinely tested).

    Protocol

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Publish Comment

Background Introduction

CD45 is a glycoprotein with protein phosphatase activity abundantly expressed on the surface of all leukocytes. The protein is highly preserved during evolution and has been demonstrated even in chicken, sharks, and mosquitos. CD45 increases T-cell and B-cell receptor signaling as well as the development and function of leukocytes. Moreover, CD45 can reduce Toll-like receptors and cytokine signaling, as well as cell adhesion and migration in a cell type specific manner. In humans, six splice variants differing in the extracellular part of the molecule have been demonstrated. The human variants are termed CD45RABC (also named CD45R), CD45RAB, CD45RBC, CD45RA, CD45RB, and CD45RO, and are detected by subtype specific antibodies and molecular weight, respectively. CD45RO is expressed on most thymocytes, activated T cells, memory T cells, granulocytes and monocytes, but only on a proportion of resting T cells.

Frontier Progress

 
Drug Development Progress
  • English Name:

    Leukocyte common antigen

  • Category:

  • Listed Drugs Count:

    0 Details

  • Clinical Drugs Count:

    4 Details

  • Highest R&D Stage:

    Phase 3 Clinical

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