Biotinylated Virus-Like Particle (VLP) Protein Isotype Control

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Menge
VLP-N82P3-30ug
$125.00
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VLP-N82P3-150ug
$410.00
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VLP-N82P3
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Application
  • Immunization
  • Cell based assay (FACS or functional assay)
  • Screening (ELISA)
Synonyms

VLP, Virus Like Particle, eVLP, VLPs

Source

Biotinylated Virus-Like Particle (VLP) Protein Isotype Control (VLP-N82P3) is expressed from human 293 cells (HEK293).

Molecular Characterization

This protein carries no "tag".

*The isotype control of empty/mock VLP (Cat. No. VLP-N82P3) is sold separately and not included in protein, you can follow the link for product information.

Structure

Virus-like particles (VLPs) are protein particles that mimic the shape of viruses but lack a viral genome, and incorporating transmembrane proteins on the external surface.

Synthesis Process

Envelope virus-like particles (eVLPs) are produced by co-expressing target membrane proteins with viral structural proteins in mammalian cells, enabling native embedding of membrane proteins into the lipid bilayer during VLP budding. This approach preserves authentic conformation, post-translational modifications, and orientation, while creating a completely detergent-free environment with repetitive multivalent display and strong immunogenicity making them ideal for immunization, binding assays in antibody discovery and screening (ELISA), and cell-based assays such as FACS, etc.

Labeling

The VLP is biotinylated with proprietary technology.

Protein Ratio

Passed as determined by the HABA assay / binding ELISA.

Formulation

The VLPs are highly immunogenic, so the immunization strategy should be optimized (antigen dose, regimen and adjuvant).

Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Shipping and Storage

This product is supplied and shipped on dry ice.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • The product MUST be stored at -70°C or lower upon receipt;
  • -70°C for 12 months under sterile conditions.

Background Introduction

Virus-like particles (VLPs) are highly structured protein particles composed of single or multiple structural proteins of the virus. Its morphological structure is similar to that of natural virus particles, does not contain virus accounting, and has strong immunogenicity and biological activity. The empty shell structure of most VLP is icosahedral or helical structure, which is composed of several specific structural proteins. At present, it is widely used in vaccine production and antigen presentation.