Biotinylated Human VEGF165 Protein, epitope tag free, ultra sensitivity (primary amine labeling) (MALS verified)

RP1-261G23.1, MGC70609, MVCD1, VEGFA, VPF

Biotinylated Human VEGF165, epitope tag free, primary amine labeling (VE5-H8210) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4). In the region Ala 27 - Arg 191, the amino acid sequences of VEGF165 from Human, Cynomolgus and Rhesus macaque are identical. It is the biotinylated form of Human VEGF165, premium grade (Cat. No. VE5-H4210).

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

Passed as determined by the HABA assay / binding ELISA.

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

• -20°C to -70°C for 24 months in lyophilized state;
• -70°C for 24 months under sterile conditions after reconstitution.