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Product Details
Synonyms
FIGF, VEGFD
Source
Biotinylated Cynomolgus VEGF-D Protein, His,Avitag (VED-C82E3) is expressed from human 293 cells (HEK293). It contains AA Phe 93 - Ser 201 (Accession # G7Q297).
Predicted N-terminus: Phe 93
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 19.7 kDa. The protein migrates as 27-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Biotinylated Cynomolgus VEGF-D Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Biotinylated Cynomolgus VEGF-D Protein, His,Avitag (Cat. No. VED-C82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) with a linear range of 0.005-0.156 μg/mL (QC tested).
Protocol
Bioactivity-SPR
Human VEGF R3, Fc Tag (Cat. No. FL4-H5251) captured on Protein A Chip can bind Biotinylated Cynomolgus VEGF-D Protein, His,Avitag (Cat. No. VED-C82E3) with an affinity constant of 1.19 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-CELL BASE
Biotinylated Cynomolgus VEGF-D Protein, His,Avitag (Cat. No. VED-C82E3) induced NFAT-Luc-VEGF R2 Reporter activity in HEK293 cells. The EC50 value of this effect is 1.42 μg/mL (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Vascular endothelial growth factor D
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
2 Details
- Highest R&D Stage：
Phase 2 Clinical
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