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Product Details
Synonyms
DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNFα
Source
Biotinylated Cynomolgus TNF-alpha Protein, His,Avitag, active trimer (TNA-C82E3) is expressed from human 293 cells (HEK293). It contains AA Val 77 - Leu 233 (Accession # P79337).
Predicted N-terminus: Val 77
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 20.8 kDa. The protein migrates as 22-24 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a trimer.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Biotinylated Cynomolgus TNF-alpha Protein, His,Avitag, active trimer on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Cynomolgus TNF-alpha Protein, His,Avitag, active trimer (Cat. No. TNA-C82E3) is more than 90% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Cynomolgus / Rhesus macaque TNFR2, His Tag (Cat. No. TN2-C52H7) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus TNF-alpha Protein, His,Avitag, active trimer (Cat. No. TNA-C82E3) with a linear range of 0.06-2 ng/mL (QC tested).
Protocol
Immobilized Human TNFR1, Fc Tag (Cat. No. TN1-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus TNF-alpha Protein, His,Avitag, active trimer (Cat. No. TNA-C82E3) with a linear range of 0.06-2 ng/mL (Routinely tested).
Protocol
User Reviews Publish Comment
Frontier Progress
Drug Development Progress- English Name：
Tumor necrosis factor α
- Category：
- Listed Drugs Count：
68 Details
- Clinical Drugs Count：
74 Details
- Highest R&D Stage：
Approved
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