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Product Details
Synonyms
TL1A, VEGI, TNFSF15
Source
Biotinylated Cynomolgus/Rhesus macaque TL1A Protein, Avitag,His Tag (TLA-C82E3) is expressed from human 293 cells (HEK293). It contains AA Leu 72 - Leu 251 (Accession # F6S8F9-1). In the region Leu 72 - Leu 251, the amino acid sequences of Cynomolgus and Rhesus macaque TL1A are identical.
Predicted N-terminus: Gly
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 23.7 kDa. The protein migrates as 26 kDa, 30 kDa and 33 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a trimer.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the SABA assay / HABA assay / binding ELISA.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Data Display
SDS-PAGE
Biotinylated Cynomolgus/Rhesus macaque TL1A Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Cynomolgus/Rhesus macaque TL1A Protein, Avitag,His Tag (Cat. No. TLA-C82E3) is more than 90% and the molecular weight of this protein is around 70-90 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Cynomolgus/Rhesus macaque TL1A Protein, Avitag,His Tag (Cat. No. TLA-C82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human DR3 Protein, Fc Tag (Cat. No. DR3-H5253) with a linear range of 5-78 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Cynomolgus/Rhesus macaque TL1A Protein, Avitag,His Tag (Cat. No. TLA-C82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human DcR3, Fc Tag (Cat. No. TNB-H5255) with a linear range of 0.6-10 ng/mL (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Tumor necrosis factor superfamily member 15
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
18 Details
- Highest R&D Stage：
Phase 3 Clinical
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