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Product Details
Synonyms
betaKlotho, beta-klotho, BKL, KLB, klotho beta like, klotho beta-like protein
Source
Biotinylated Human Klotho beta Protein, His Tag, primary amine labeling (KLB-H82H3) is expressed from human 293 cells (HEK293). It contains AA Met 30 - Leu 997 (Accession # Q86Z14-1).
Predicted N-terminus: Met 30
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 112.9 kDa. The protein migrates as 130-150 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Biotinylated Human Klotho beta Protein, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human FGF19, Fc Tag (Cat. No. FG9-H5253) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Klotho beta Protein, His Tag, primary amine labeling (Cat. No. KLB-H82H3) with a linear range of 0.02-0.625 μg/mL (QC tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Klotho beta
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
3 Details
- Highest R&D Stage：
Phase 2 Clinical
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