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Integrin alpha V beta 6, ITGAV&ITGB6
Biotinylated Cynomolgus ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free (IT6-C82E3) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Pro 993 & Gly 22 - Pro 709 (Accession # A0A2K5WCD3-1 & A0A2K5TZ36-1).
Predicted N-terminus: Phe 31 & Gly 22
Biotinylated Cynomolgus ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of ITGAV and ITGB6, has a calculated MW of 114.8 kDa (ITGAV) & 79.9 kDa (ITGB6). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB6 contains no tag but a basic tail at the C-terminus. The protein migrates as 130-150 kDa (ITGAV) and 80-95 kDa (ITGB6) when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the SABA assay / HABA assay / binding ELISA.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.