Biotinylated Cynomolgus Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)

Synonyms

Integrin alpha V beta 3, ITGAV&ITGB3

Source

Biotinylated Cynomolgus ITGAV&ITGB3 Heterodimer Protein, His,Avitag&Tag Free (IT3-C82W3) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Pro 993 | Gly 27 - Asp 718 (Accession # A0A2K5WCD3-1 | A0A2K5WYN9).

Predicted N-terminus: Phe 31 | Gly 27

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

The protein has a calculated MW of 115.2 kDa | 81.8 kDa. The protein migrates as 95-100 kDa, 120-130 kDa and 140-160 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

The protein is designed as a dimer.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the HABA assay / binding ELISA.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process