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Product Details
Synonyms
IL33, DV27, C9ORF26, IL1F11, NFHEV, DKFZp586H0523, DVS27, NFEHEV, RP11-575C20.2
Source
Biotinylated Cynomolgus IL-33 Protein, His,Avitag (IL3-C82E3) is expressed from human 293 cells (HEK293). It contains AA His 109 - Ile 270 (Accession # A0A2K5W3I1-1).
Predicted N-terminus: His 109
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 21.9 kDa. The protein migrates as 27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Biotinylated Cynomolgus IL-33 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Biotinylated Cynomolgus IL-33 Protein, His,Avitag (Cat. No. IL3-C82E3) was greater than 95% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Human IL-1RL1, Fc Tag (Cat. No. IL1-H5250) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus IL-33 Protein, His,Avitag (Cat. No. IL3-C82E3) with a linear range of 0.2-2 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Cynomolgus IL-33 Protein, His,Avitag (Cat. No. IL3-C82E3) on SA Biosensor, can bind Human IL-1RL1, Fc Tag (Cat. No. IL1-H5250) with an affinity constant of 2.97 nM as determined in BLI assay (ForteBio Octet RED96e) (Routinely tested).
Protocol
Loaded Human IL-1RL1, Fc Tag (Cat. No. IL1-H5250) on Protein A Biosensor, can bind Biotinylated Cynomolgus IL-33 Protein, His,Avitag (Cat. No. IL3-C82E3) with an affinity constant of 9.68 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
User Reviews Publish Comment
Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Interleukin-33
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
9 Details
- Highest R&D Stage：
Phase 3 Clinical
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