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Product Details
Synonyms
Interleukin-22 receptor subunit alpha-1, IL-22 receptor subunit alpha-1, IL-22R-alpha-1, IL-22RA1, Cytokine receptor class-II member 9, Cytokine receptor family 2 member 9, CRF2-9, ZcytoR11, IL22RA1 , IL22R
Source
Biotinylated Human IL-22 R alpha 1 Superstable Mutant Protein, His,Avitag (IL1-H82E5) is expressed from human 293 cells (HEK293). It contains AA Pro 18 - Thr 228 (Accession # Q8N6P7).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 27.8 kDa. The protein migrates as 35 kDa and 37-41 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-HPLC.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Biotinylated Human IL-22 R alpha 1 Superstable Mutant Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-HPLC
The purity of Biotinylated Human IL-22 R alpha 1 Superstable Mutant Protein, His,Avitag (Cat. No. IL1-H82E5) was greater than 95% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Human IL-22, His Tag (Cat. No. IL2-H524a) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-22 R alpha 1 Superstable Mutant Protein, His,Avitag (Cat. No. IL1-H82E5) with a linear range of 5-156 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human IL-22 R alpha 1 Superstable Mutant Protein, His,Avitag (Cat. No. IL1-H82E5) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Temtokibart with a linear range of 0.06-2 ng/mL (Routinely tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Interleukin-22 receptor
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
5 Details
- Highest R&D Stage：
Phase 2 Clinical
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