Biotinylated Human IL-17 RA / CD217 Protein, His,Avitag™

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Cat. No. / Size
Price
Qty
ILR-H82E5-25ug
$360.00
ILR-H82E5-200ug
$1565.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    CD217, CDw217, IL-17RA, IL17R, CANDF5, hIL-17R

  • Source

    Biotinylated Human IL-17 RA, His,Avitag (ILR-H82E5) is expressed from human 293 cells (HEK293). It contains AA Leu 33 - Trp 320 (Accession # Q96F46-1).

    Predicted N-terminus: Leu 33

    Request for sequence
  • Molecular Characterization

    IL-17 RA Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 37.2 kDa. The protein migrates as 50-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    IL-17 RA SDS-PAGE

    Biotinylated Human IL-17 RA, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity-ELISA

     IL-17 RA ELISA

    Immobilized Human IL17A, Tag Free (Cat. No. ILA-H5219) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-17 RA, His,Avitag (Cat. No. ILR-H82E5) with a linear range of 0.2-6.25 ng/mL (QC tested).

    Protocol

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Background

Interleukin 17 receptor A (IL17RA) is also known as cluster of differentiation w217 (CDw217), is a pro-inflammatory cytokine secreted by activated T-lymphocytes, belong to ubiquitous type I membrane glycoprotein, and binds with low affinity to interleukin 17A (IL17A). IL 17R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested . IL 17RA associates with IL 17RC to form a signaling receptor complex for IL 17 and IL 17F . Ligand and IL 17RA ligation promotes T cell activation and the production of IL - 6, G-CSF, SCF, and multiple pro-inflammatory chemokines. Defects in IL17RA are the cause of familial candidiasis type 5 (CANDF5).

Recent Advances

 
Drug Development Progress
  • English Name:

    Interleukin-17 receptor A

  • Category:

  • Approved Drugs:

    1 Details

  • Drugs in Clinical Trials:

    2 Details

  • Highest Development Stage:

    Approved

Drug Candidate Licensing
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  • Background