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Product Details
Synonyms
IL13, ALRH, BHR1, MGC116786, MGC116788, MGC116789, P600, Interleukin-13
Source
Biotinylated Cynomolgus IL-13 Protein, His,Avitag (IL3-C82E4) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Asn 132 (Accession # ABG75889.1).
Predicted N-terminus: Ser 21
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 15.9 kDa. The protein migrates as 13-15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Cynomolgus IL-13 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Cynomolgus IL-13 Protein, His,Avitag (Cat. No. IL3-C82E4) is more than 95% and the molecular weight of this protein is around 15-20 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human IL-13 R alpha 2 Protein, Fc Tag (Cat. No. IL2-H5256) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus IL-13 Protein, His,Avitag (Cat. No. IL3-C82E4) with a linear range of 0.005-1 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Cynomolgus IL-13 R alpha 1, His Tag (Cat. No. IL1-C52H8) immobilized on CM5 Chip can bind Biotinylated Cynomolgus IL-13 Protein, His,Avitag (Cat. No. IL3-C82E4) with an affinity constant of 24.6 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-13
- Category：
- Approved Drugs：
4 Details
- Drugs in Clinical Trials：
27 Details
- Highest Development Stage：
Approved
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