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Product Details
Synonyms
IFNB1, Interferon beta, IFN-beta, IFB, IFNB
Source
Biotinylated Human IFN-beta Protein, His,Avitag (IFB-H82E4) is expressed from human 293 cells (HEK293). It contains AA Met 22 - Asn 187 (Accession # P01574).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 23.9 kDa. The protein migrates as 27-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Biotinylated Human IFN-beta Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human IFN-beta Protein, His,Avitag (Cat. No. IFB-H82E4) is more than 90% and the molecular weight of this protein is around 25-35 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Biotinylated Human IFN-beta Protein, His,Avitag (Cat. No. IFB-H82E4) captured on Biotin CAP-Series S Sensor Chip can bind Human IFNAR2, His Tag (Cat. No. IF2-H5224) with an affinity constant of 203 nM as determined in a SPR assay (Biacore 8K) (QC tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Interferon beta
- Category：
- Listed Drugs Count：
1 Details
- Clinical Drugs Count：
6 Details
- Highest R&D Stage：
Approved
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