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Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*15:01&MBP (ENPVVHFFKNIVTPR) Complex Protein (Monomer, MALS verified)

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Menge
HLM-H82Ea-25ug
$120.00
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HLM-H82Ea-200ug
$680.00
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HLM-H82Ea
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Synonyms

HLA-DRA1*0101 & HLA-DRB1*1501 & MBP(ENPVVHFFKNIVTPR)

Source

Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*15:01&MBP (ENPVVHFFKNIVTPR) Complex Protein (HLM-H82Ea) is expressed from human 293 cells (HEK293). It contains AA Ile 26 - Glu 216 (HLA-DRA1*01:01) & Gly 30 - Lys 227 (HLA-DRB1*15:01) & ENPVVHFFKNIVTPR peptide (Accession # CAI2388006.1 (HLA-DRA1*01:01) & CAI2388008.1 (HLA-DRB1*15:01) & ENPVVHFFKNIVTPR).

Predicted N-terminus: Ile 26 & Glu

Molecular Characterization

Biotinylated Human HLA-DRA1*01:01&HLA-DRB1*15:01&MBP (ENPVVHFFKNIVTPR) Complex Protein is produced by co-expression of HLA-DRA1 and HLA-DRB1 loaded with MBP peptide.

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 29.8 kDa and 29.2 kDa. The protein migrates as 37-40 kDa and 32-37 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background Introduction

The peptide ENPVVHFFKNIVTPR, derived from myelin basic protein (MBP), is presented by the MHC class II heterodimer HLA-DRA1*01:01/HLA-DRB1*15:01. This specific peptide-MHC II complex is a predominant autoantigen target in multiple sclerosis (MS) and is strongly associated with the HLA-DRB1*15:01 genetic risk allele. Its recognition by autoreactive CD4⁺ T helper cells initiates and drives the inflammatory immune response against the myelin sheath in the central nervous system, making it a prototypical complex for studying autoimmune demyelination and MS pathogenesis.