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Biotinylated Human HLA-A*02:01&B2M&Survivin (LTLGEFLKL) Complex Protein (Monomer, MALS verified)

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HLN-H82E7-25ug
$120.00
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HLN-H82E7-200ug
$680.00
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HLN-H82E7
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Product Details
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  • Product Details
  • Performance Data
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Synonyms

HLA-A*0201 & B2M & Survivin (LTLGEFLKL)

Source

Biotinylated Human HLA-A*02:01&B2M&Survivin (LTLGEFLKL) Complex Protein (HLN-H82E7) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & LTLGEFLKL peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & LTLGEFLKL).

Predicted N-terminus: Gly 25 & Ile 21

Molecular Characterization

Biotinylated Human HLA-A*02:01&B2M&Survivin (LTLGEFLKL) Complex Protein is produced by co-expression of HLA and B2M loaded with Survivin peptide.

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 40-43 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Purity

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

The HLA-A*02:01&B2M&Survivin (LTLGEFLKL) complex is a key antigen-presenting complex in the immune system. HLA-A*02:01, a major histocompatibility complex (MHC) class I molecule, forms a stable heterodimer with β2-microglobulin (B2M). Subsequently, this dimer binds to the Survivin-derived peptide LTLGEFLKL—a specific epitope of Survivin, an anti-apoptotic protein overexpressed in various tumors. This complex is critical for activating cytotoxic T lymphocytes (CTLs), enabling the immune system to recognize and eliminate tumor cells expressing Survivin.