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HLA-A*0201 & B2M & insB (HLVEALYLV)
Biotinylated Human HLA-A*02:01&B2M&insB (HLVEALYLV) Complex Protein (HLB-H82E4) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & HLVEALYLV peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & insB (HLVEALYLV)).
Predicted N-terminus: Gly 25 & His
Biotinylated Human HLA-A*02:01&B2M&insB (HLVEALYLV) Complex Protein is produced by co-expression of HLA-A*0201 and B2M loaded with insB peptide.
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 36.4 kDa and 13.8 kDa. The protein migrates as 40-43 kDa and 12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.