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Product Details
Synonyms
MET, AUTS9, HGFR, RCCP2, c-Met
Source
Biotinylated Human HGF R Protein, His,Avitag (MET-H82E3) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Asp 741 (Accession # P08581-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 84.4 kDa. The protein migrates as 60-70 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Biotinylated Human HGF R Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Biotinylated Human HGF R Protein, His,Avitag (Cat. No. MET-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human HGF, Fc Tag (Cat. No. HGF-H5253) with a linear range of 0.2-2 ng/mL (QC tested).
Protocol
Immobilized Human HGF, Fc Tag (Cat. No. HGF-H5253) at 2 μg/mL (100 μL/well) can bind Biotinylated Human HGF R Protein, His,Avitag (Cat. No. MET-H82E3) with a linear range of 1-31 ng/mL (Routinely tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Hepatocyte growth factor receptor
- Category：
- Listed Drugs Count：
13 Details
- Clinical Drugs Count：
74 Details
- Highest R&D Stage：
Approved
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