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Product Details
Synonyms
Glypican 2, GPC2
Source
Biotinylated Rhesus macaque Glypican 2 Protein, His,Avitag (GP2-R82E3) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Ser 552 (Accession # XP_001103286).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 61.6 kDa. The protein migrates as 30-33 kDa and 35-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Data Display
SDS-PAGE
Biotinylated Rhesus macaque Glypican 2 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Rhesus macaque Glypican 2 Protein, His,Avitag (Cat. No. GP2-R82E3) is more than 85% and the molecular weight of this protein is around 50-80 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Midkine Protein, premium grade (Cat. No. MIE-H5117) at 5 μg/mL (100 μL/well) can bind Biotinylated Rhesus macaque Glypican 2 Protein, His,Avitag (Cat. No. GP2-R82E3) with a linear range of 0.001-0.5 μg/mL (QC tested).
Protocol
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Background Introduction
Frontier Progress
Drug Development Progress- English Name：
Glypican-2
- Category：
- Listed Drugs Count：
0 Details
- Clinical Drugs Count：
0 Details
- Highest R&D Stage：
Phase 1 Clinical
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